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Updated: Oct 16, 2026

Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae
Published on: April 11, 2021
Transcriptional and post-transcriptional in organello labelling of Trypanosoma brucei mitochondrial RNA
K T Militello1, M L Hayman, L K Read
1Department of Microbiology and Center for Microbial Pathogenesis, SUNY at Buffalo School of Medicine, Buffalo, NY 14214, USA.
Abstract:
In organello labelling of Trypanosoma brucei mitochondrial (mt) RNA was characterised with respect to nucleotide requirements and drug sensitivity. Mitochondrial transcriptional activity is maximal in the presence of all ribonucleoside-triphosphate NTPs, and can be inhibited by UTP depletion. Mitochondrial transcription can also be partially inhibited by actinomycin D (actD) or ethidium bromide (EtBr). Post-transcriptional UTP incorporation is insensitive to actinomycin D or ethidium bromide. Proteins were identified that interact with transcriptional and post-transcriptionally labelled RNAs, and confirm the in vitro RNA-binding properties discovered for a number of T. brucei mt proteins. These experiments reveal new strategies for studying mt transcription and processing in T. brucei mitochondria.
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