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Prevention of cross-contamination during sampling procedure in molecular detection for cancer micrometastasis
1Department of Surgery and Clinical Oncology, Graduate School of Medicine, Osaka University, 2-2 Yamada-Oka, Suita, Osaka, Japan. fujiwara@surg2.med.osaka-u.ac.jp
Abstract:
Reverse transcriptase-polymerase chain reaction (RT-PCR) techniques have been widely employed as an ultra-sensitive method for detection of micrometastases in patients with various types of malignancies. Messenger RNA of a specific marker gene is a target for RT-PCR amplification to examine the presence of micrometastases in body fluids or tissues obtained from human. We developed the RT-PCR assay specific for rat beta-actin mRNA, which cannot detect human counterpart and assessed how much contamination of rat tissues can influence the result of RT-PCR assay and how to avoid the influence of the contamination in RT-PCR assay.