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Related Experiment Videos

Ca2+ fluxes in developing Trichoderma viride mycelium.

M Simkovic1, S Krystofová, L Varecka

  • 1Department of Biochemistry and Microbiology, Slovak University of Technology, Bratislava, Slovak Republic.

Canadian Journal of Microbiology
|April 26, 2000
PubMed
Summary
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Calcium (Ca2+) transport in Trichoderma viride mycelium involves distinct influx and efflux systems. These systems are developmentally regulated, with Ca2+ influx crucial in early growth stages and diminishing later.

Area of Science:

  • Mycology
  • Cell Biology
  • Biochemistry

Background:

  • Calcium ions (Ca2+) play critical roles in fungal development and cellular processes.
  • Understanding Ca2+ transport mechanisms is essential for characterizing fungal physiology.
  • Trichoderma viride is a model organism for studying fungal growth and interactions.

Purpose of the Study:

  • To investigate the characteristics of Ca2+ influx and efflux in Trichoderma viride mycelium throughout its life cycle.
  • To elucidate the transport systems involved in Ca2+ homeostasis.
  • To determine the developmental regulation of Ca2+ transport.

Main Methods:

  • Utilized radioactive 45Ca2+ tracer experiments to quantify Ca2+ fluxes.
  • Employed X-ray fluorescence spectroscopy for elemental analysis.

Related Experiment Videos

  • Investigated effects of chemical agents (uncouplers, azalomycin F, salicylate, A23187) on Ca2+ transport and organelle Ca2+ content.

    • Main Results:

    • Ca2+ influx and efflux are mediated by separate transport systems, potentially involving Ca2+/nH+ antiport or Ca2+ uniport.
    • Uncouplers, azalomycin F, and salicylate differentially affected Ca2+ influx and efflux.
    • Ca2+ influx peaked at 30 hours of cultivation and decreased significantly in later stages, indicating developmental downregulation.

    Conclusions:

    • The capacitative Ca2+ influx mechanism may explain the observed transport properties.
    • Ca2+ uptake is primarily active during the early developmental stages of Trichoderma viride mycelium.
    • Later-stage mycelial growth appears independent of extracellular Ca2+ until conidiation.