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[Transformation of Mycoplasma hominis by plasmid pAM120 using electroporation]
N M Aleksandrova1, M R Bevova, V M Govorun
1Research Institute of Physicochemical Medicine, Moscow, Russia.
Genetika
|April 26, 2000
Abstract:
Mycoplasma hominis was transformed by electroporation with plasmid pAM120 containing the transposon Tn916 that carried the tetM gene responsible for the resistance to tetracycline. The frequency of transformation was 10(-7)-10(-8) colony-forming units (CFU) per 10 micrograms of plasmid DNA. The PCR analysis of transformed DNA confirmed the transposon integration into the mycoplasma genome.