[Possibilities of selection with an increase in frequency of meiotic recombination (simulation and experiment)]

Polymorphism of rec genes, modifier genes of recombination frequency (rf), was analyzed. Genetic population analysis was used to estimate the efficiency of some schemes of direct and indirect selection for higher rf in a marked chromosomal region. A new modification of the cyclic selection scheme, based on selection of double recombinant marker genotypes in the offspring of self-fertilized plants, was found to be most effective in many cases. This indirect selection scheme was studied in more detail using deterministic and stochastic computer simulation. Effects of various factors on the efficiency of the modified scheme were analyzed. These factors were different degrees of cross-fertilization, differential adaptation of recombinant and non-recombinant gametes and zygotes, linkage between marker genes and rec genes, and environmental variation of rf. Sample sizes and the number of crosses required for a stable rf increase in experiment were estimated. Statistical properties of two new modified tests for comparison of rf values were studied. An experiment with marker forms of tomato were conducted taking into account the recommendations following from the results of simulation.