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[ATP and polyphosphate-dependent bacterial NAD+-kinases].
S Iu Filippovich1, T P Afanas'eva, G P Bachurina
1Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia.
Prikladnaia Biokhimiia I Mikrobiologiia
|April 26, 2000
Summary
Micrococcus luteus and Corynebacterium ammoniagenes NAD+ kinases utilize inorganic tripolyphosphate, unlike E. coli. M. luteus is a promising organism for NADP production due to this unique capability.
Area of Science:
- Biochemistry
- Enzymology
- Microbiology
Context:
- NAD+ kinases are crucial enzymes in NADP biosynthesis.
- Exploring alternative substrates for NAD+ kinases is important for biotechnological applications.
- Inorganic tripolyphosphate (PPP(i)) as an ATP substitute is investigated.
Purpose:
- To investigate the substrate specificity of NAD+ kinases from different bacterial species.
- To identify microorganisms capable of utilizing PPP(i) for NADP synthesis.
- To evaluate the potential of M. luteus as an NADP producer.
Summary:
- NAD+ kinases from Micrococcus luteus and Corynebacterium ammoniagenes showed activity with PPP(i), unlike the enzyme from Escherichia coli.
- Multiple isoforms of NAD+ kinase were identified in M. luteus, C. ammoniagenes, and E. coli.
- M. luteus exhibited NADP-synthesizing activity using both ATP and PPP(i), highlighting its potential for NADP production.
Impact:
- Identifies M. luteus as a promising organism for NADP production using PPP(i).
- Expands understanding of NAD+ kinase substrate diversity in bacteria.
- Offers potential for novel biotechnological routes to NADP synthesis.