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The thrombogram: monitoring thrombin generation in platelet-rich plasma
H C Hemker1, P L Giesen, M Ramjee
1Department of Biochemistry and Synapse BV, Cardiovascular Research Institute (CARIM) Maastricht, The Netherlands. Coen.Hemker@thrombin.com
Thrombosis and Haemostasis
|April 26, 2000
Abstract:
A method is described in which thrombin activity in clotting plasma can be monitored through the continuous measurement of the fluorescent split-product of the substrate Z-Gly-Gly-Arg-AMC. The signal is not impaired by turbidity; therefore proper measurement is not disturbed by the occurrence of a clot or the presence of platelets and direct measurement in platelet rich plasma is possible.