Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA hybridization sensor utilizing [Os(5,6-dmphen)3]2+ by square wave voltammetry.

K Maruyama1, J Motonaka, Y Mishima

  • 1Department of Chemical Science and Technology, The University of Tokushima, Japan.

Nucleic Acids Symposium Series
|April 26, 2000
PubMed
Summary

This study demonstrates sensitive DNA detection using tris(5,6-dimethyl-phenanthroline) osmium(II/III) ([Os(5,6-dmphen)3]2+) as a hybridization indicator. The method achieves selective target DNA identification within a wide concentration range.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Alterations in serum levels of trace elements in tuberculosis and HIV infections.

European journal of clinical nutrition·2005
Same author

Electrochemical characterization and DNA-binding property of dipyridophenazine complexes of osmium (II).

Nucleic acids symposium series·2003
Same author

DNA electrochemical sensor using osmium complex.

Nucleic acids research. Supplement (2001)·2003
Same author

Evaluation of osmium(II) complexes as electron transfer mediators accessible for amperometric glucose sensors.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2001
Same author

Interaction between tris(bpyl)osmium(II/III) complex and immobilized DNA on a carbon electrode.

Nucleic acids symposium series·1997
Same author

Recognition of DNA sequence utilizing osmium complex by amperometric technique.

Nucleic acids symposium series·1997

Area of Science:

  • Electrochemistry
  • Analytical Chemistry
  • Biotechnology

Background:

  • DNA hybridization is crucial for molecular diagnostics.
  • Osmium phenanthroline complexes offer unique electrochemical properties.
  • [Os(5,6-dmphen)3]2+ exhibits high association constants and current density.

Purpose of the Study:

  • To investigate the use of [Os(5,6-dmphen)3]2+ as an indicator for DNA hybridization detection.
  • To establish the sensitivity and selectivity of this electrochemical method.

Main Methods:

  • Utilizing tris(5,6-dimethyl-phenanthroline) osmium(II/III) as a hybridization indicator.
  • Employing square wave voltammetry for electrochemical analysis.
  • Optimizing conditions for selective DNA detection.

Related Experiment Videos

Main Results:

  • Selective detection of target DNA was achieved.
  • The method demonstrated sensitivity over a range of 6.9 x 10(-10) to 6.9 x 10(-5) g/mL.
  • [Os(5,6-dmphen)3]2+ proved effective due to its favorable electrochemical characteristics.

Conclusions:

  • [Os(5,6-dmphen)3]2+ is a viable indicator for sensitive and selective DNA hybridization detection.
  • Square wave voltammetry coupled with this osmium complex offers a promising analytical tool for DNA analysis.