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Mechanism of ribosome RNA apurinic site specific lyase
T Sawasaki1, R Morishita, A Ozawa
1Department of Applied Chemistry, Faculty of Engineering, Ehime University, Matsuyama, Japan.
Nucleic Acids Symposium Series
|April 26, 2000
Abstract:
A new enzyme, which we named ribosome RNA apurinic site specific lyase (RALyase), has been characterized. The enzyme specifically cleaves a phosphodiester bond at the apurinic site in the sarcin/ricin domain of 28S rRNA in ribosomes. The cut ends of wheat 28S rRNA were determined as 5'---GUACG-alpha-hydroxy-alpha, beta-unsaturated aldehyde and pGAGGA---3' for the 3' fragment, demonstrating that the enzyme catalyzes the beta-elimination reaction.