Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA structures recognized by the human UV-DDB protein.

T Mizukoshi1, Y Fujiwara, S Iwai

  • 1Department of Bioorganic Chemistry, Biomolecular Engineering Research Institute, Suita, Osaka, Japan.

Nucleic Acids Symposium Series
|April 26, 2000
PubMed
Summary

The human UV-damaged DNA-binding (UV-DDB) protein binds DNA by inducing a significant bend. This protein facilitates DNA repair by recognizing and bending damaged DNA sites.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of vasopressin delivery via the proximal humerus and proximal tibia in healthy dogs under general anesthesia: implications for emergency intraosseous administration.

Polish journal of veterinary sciences·2025
Same author

Is lung resection appropriate for late octogenarians? Surgical outcomes of patients aged ≥ 80 years with lung cancer.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2021
Same author

Petahertz non-linear current in a centrosymmetric organic superconductor.

Nature communications·2020
Same author

Colonic microbiota is associated with inflammation and host epigenomic alterations in inflammatory bowel disease.

Nature communications·2020
Same author

Surgical approaches for condylar fractures related to facial nerve injury: deep versus superficial dissection.

International journal of oral and maxillofacial surgery·2019
Same author

Immunogenic cell death by oncolytic herpes simplex virus type 1 in squamous cell carcinoma cells.

Cancer gene therapy·2016

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • DNA damage is a critical factor in cellular mutations and disease.
  • The UV-damaged DNA-binding (UV-DDB) protein plays a role in DNA repair pathways.
  • Understanding protein-DNA interactions is crucial for deciphering cellular processes.

Purpose of the Study:

  • To characterize the DNA recognition mechanism of the human UV-DDB protein.
  • To investigate the structural changes in DNA upon binding with UV-DDB.
  • To elucidate the role of DNA bending in UV-DDB function.

Main Methods:

  • Circular permutation analyses were employed to study DNA bending.
  • Fluorescence resonance energy transfer (FRET) experiments were conducted.
  • Analysis of DNA duplexes with (6-4) photoproduct and abasic site analogs.

Main Results:

  • DNA duplexes with (6-4) photoproduct and abasic site analogs were bent by 54 and 57 degrees, respectively, upon complex formation with UV-DDB.
  • Fluorescence resonance energy transfer experiments indicated no intrinsic bend in these DNA duplexes.
  • The UV-DDB protein induces significant bending in damaged DNA substrates.

Conclusions:

  • The UV-DDB protein binds to DNA by inducing a pronounced bend.
  • This DNA bending ability is a key feature of UV-DDB's recognition mechanism.
  • UV-DDB facilitates DNA repair by interacting with and bending damaged DNA sites.

Related Experiment Videos