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Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Utero-tubal Embryo Transfer and Vasectomy in the Mouse Model
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Techniques of embryo transfer and facility decontamination used to improve the health and welfare of transgenic mice.

J M Morrell1

  • 1European Molecular Biology Laboratory, Heidelberg, Germany.

Laboratory Animals
|April 26, 2000
PubMed
Summary

Re-deriving transgenic mouse lines and maintaining them in a barrier unit significantly improves animal health and welfare. This method successfully prevents disease transmission, enhancing research reliability.

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Area of Science:

  • Animal models in biomedical research
  • Laboratory animal science
  • Transgenic mouse technology

Background:

  • Transgenic mouse studies require high standards of animal health and welfare for reliable results.
  • Current practices may not always ensure microbiological integrity, potentially impacting research outcomes.
  • Improving animal health aligns with the principles of the '3Rs' (Reduction, Refinement, Replacement) in animal experimentation.

Purpose of the Study:

  • To describe the initial steps of a project aimed at enhancing the health and welfare of transgenic mice at the European Molecular Biology Laboratory (EMBL).
  • To implement re-derivation of transgenic mouse lines into a microbiologically defined state for maintenance in a barrier facility.
  • To validate a novel sterilization protocol for barrier units that is effective and environmentally conscious.

Main Methods:

  • Re-derivation of transgenic mouse lines through embryo transfer from contaminated donors to specific pathogen-free (SPF) recipients.
  • Maintenance of re-derived lines in a newly constructed barrier animal facility with restricted access.
  • Sterilization of the barrier unit using a formaldehyde-free protocol, validated by in vitro and in vivo screening.

Main Results:

  • Successful transfer of embryos without disease carry-over from a contaminated old facility to a new barrier unit.
  • Offspring from re-derived lines were of high health status, free from pathogens and antibodies present in the old unit.
  • Sentinel animals confirmed the absence of specific murine viruses (e.g., mouse hepatitis virus) and parasites in the re-derived population.
  • The formaldehyde-free sterilization protocol proved effective in achieving microbiological sterility and was cost-effective.

Conclusions:

  • Re-deriving transgenic mouse lines and maintaining them under stringent barrier conditions is a successful strategy for disease prevention and welfare enhancement.
  • The implemented methods significantly improve the microbiological status of transgenic mouse colonies.
  • The developed sterilization protocol is a safe, effective, and economical alternative for barrier unit decontamination.