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Related Experiment Videos

TRAIL expression in vascular smooth muscle.

B R Gochuico1, J Zhang, B Y Ma

  • 1Pulmonary Center and Department of Microbiology, Boston University School of Medicine, Boston, Massachusetts 02118, USA.

American Journal of Physiology. Lung Cellular and Molecular Physiology
|April 27, 2000
PubMed
Summary

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is expressed in vascular smooth muscle cells of the pulmonary artery and aorta. TRAIL induces apoptosis and cell lysis in these vascular cells, impacting vessel wall turnover.

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Area of Science:

  • Vascular Biology
  • Cell Death Signaling

Background:

  • Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a key mediator of apoptosis.
  • Its role in vascular tissue, particularly smooth muscle cells, is not well understood.

Purpose of the Study:

  • To investigate the expression and function of TRAIL in murine and human vascular smooth muscle cells.
  • To determine TRAIL's role in vascular cell apoptosis and turnover.

Main Methods:

  • In situ hybridization and immunohistochemistry were used to detect TRAIL mRNA and protein in murine pulmonary arteries and aortas.
  • Primary human aorta and pulmonary artery smooth muscle cells, as well as endothelial cells, were cultured.
  • Cells were treated with exogenous TRAIL, and apoptosis was assessed via poly(ADP ribose) polymerase cleavage.

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Main Results:

  • TRAIL mRNA and protein were specifically localized in the medial smooth muscle cell layer of murine pulmonary arteries and aortas.
  • Human aorta and pulmonary artery smooth muscle cells expressed abundant TRAIL.
  • Exogenous TRAIL induced cell lysis and apoptosis in smooth muscle and endothelial cells, confirmed by PARP cleavage.

Conclusions:

  • TRAIL is expressed in vascular smooth muscle cells and contributes to apoptosis in the vessel wall.
  • These findings provide insights into the regulation of cell turnover in vascular tissues.
  • TRAIL may play a significant role in vascular homeostasis and disease.