Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cyclodextrin-peptide hybrid as a hydrolytic catalyst having multiple functional groups.

H Tsutsumi1, K Hamasaki, H Mihara

  • 1Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.

Bioorganic & Medicinal Chemistry Letters
|April 27, 2000
PubMed
Summary

A novel cyclodextrin peptide hybrid was synthesized to catalyze ester hydrolysis. Kinetic analysis confirmed the crucial role of the carboxylate group in this catalytic system.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comprehensive analysis of gene expressions in ileal mucosa of chickens fed paddy rice and their IgA response to oral vaccination.

Poultry science·2025
Same author

Association of subpleural ground-glass opacities with respiratory failure and RNAemia in COVID-19.

European radiology·2023
Same author

Epstein-Barr virus-associated inflammatory pseudotumor variant of follicular dendritic cell sarcoma of the liver: a case report and review of the literature.

Surgical case reports·2022
Same author

Gastrointestinal: Pembrolizumab-induced gastric ulcer occurring as an immune-related adverse event.

Journal of gastroenterology and hepatology·2022
Same author

Clinic-level factors associated with collaboration with community pharmacies in Japan.

Die Pharmazie·2018
Same author

Ingestion of paddy rice increases intestinal mucin secretion and goblet cell number and prevents dextran sodium sulfate-induced intestinal barrier defect in chickens.

Poultry science·2018

Area of Science:

  • Supramolecular Chemistry
  • Catalysis
  • Organic Chemistry

Background:

  • Cyclodextrins are cyclic oligosaccharides with a hydrophobic cavity and hydrophilic exterior, widely used in host-guest chemistry.
  • Peptide-based catalysts offer tunable structures and functionalities for various chemical transformations.
  • Combining cyclodextrins and peptides can lead to synergistic effects in catalysis.

Purpose of the Study:

  • To design and synthesize a novel cyclodextrin-peptide hybrid molecule.
  • To investigate the catalytic activity of this hybrid in ester hydrolysis.
  • To elucidate the role of specific functional groups within the hybrid in the catalytic mechanism.

Main Methods:

  • Synthesis of a cyclodextrin-peptide hybrid incorporating an alpha-helix peptide backbone.

Related Experiment Videos

  • Characterization of the synthesized hybrid using spectroscopic techniques.
  • Kinetic studies to evaluate the catalytic efficiency for ester hydrolysis.
  • Analysis of reaction kinetics to determine the rate-limiting steps and identify key catalytic species.

    • Main Results:

    • Successful synthesis of a functionalized cyclodextrin-peptide hybrid.
    • Demonstration of catalytic activity in ester hydrolysis.
    • Kinetic data indicated that the carboxylate group is essential for the observed catalytic activity.
    • The alpha-helix structure of the peptide backbone likely contributes to substrate binding and orientation.

    Conclusions:

    • The designed cyclodextrin-peptide hybrid serves as an effective catalyst for ester hydrolysis.
    • The carboxylate group plays a pivotal role in the catalytic mechanism, likely through acid-base catalysis.
    • This work highlights the potential of integrating supramolecular hosts with peptides for developing advanced catalytic systems.