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Updated: Oct 16, 2026
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Published on: January 2, 2017
Pretreatment with MK-801 or the lazaroid U-83836E does not enhance striatal graft survival
U Mundt-Petersen1, J Karlsson, G S Schierle
1Department of Physiological Sciences, Wallenberg Neuroscience Center, Lund University, Sweden.
Abstract:
A large proportion of grafted striatal neurons die, and mechanisms by which they succumb may involve excitotoxicity and oxidative stress. We investigated the effects of pretreatment of the graft tissue with the N-methyl-D-aspartate (NMDA) receptor antagonist (+)dizocilpine hydrogen maleate (MK-801) and lipid peroxidation inhibitor lazaroid U-83836E on the survival of transplanted striatal neurons. Neither compound increased the survival of grafts, suggesting that NMDA-related excitotoxicity or oxidative stress may not be primary mediators of cell death in striatal grafts.
Insights
Transplanted striatal neurons often die. Pretreatment with an NMDA receptor antagonist or lipid peroxidation inhibitor did not improve graft survival, suggesting excitotoxicity and oxidative stress are not primary causes of cell death.
Area of Science:
- Neuroscience
- Neuron transplantation
- Cell survival
Background:
- Grafted striatal neurons exhibit significant cell death.
- Potential mechanisms include excitotoxicity and oxidative stress.
Purpose of the Study:
- To investigate the neuroprotective effects of NMDA receptor antagonist and lipid peroxidation inhibitor on grafted striatal neurons.
- To determine if excitotoxicity or oxidative stress are primary mediators of cell death in striatal grafts.
Main Methods:
- Pretreatment of graft tissue with (+)dizocilpine hydrogen maleate (MK-801), an NMDA receptor antagonist.
- Pretreatment with U-83836E, a lipid peroxidation inhibitor.
Main Results:
- Neither MK-801 nor U-83836E pretreatment enhanced the survival of transplanted striatal neurons.
- Graft survival rates were not significantly improved by the tested compounds.
Conclusions:
- NMDA-related excitotoxicity and oxidative stress may not be the primary factors contributing to cell death in striatal grafts.
- Further research is needed to identify the key mediators of cell death in this context.
