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Related Experiment Videos

Developmental changes in cholesterol 7alpha- and 27-hydroxylases in the piglet.

D S Lewis1, S Oren, X Wang

  • 1Department of Food Science and Human Nutrition, Iowa State University, Ames 50011, USA. dslewis@iastate.edu

Journal of Animal Science
|April 28, 2000
PubMed
Summary

Hepatic cholesterol 7alpha-hydroxylase (CYP7A) activity increases after birth in piglets, regulated by pretranslational mechanisms. Fasting significantly reduces CYP7A activity and mRNA levels, with recovery after refeeding.

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Area of Science:

  • Biochemistry
  • Developmental Biology
  • Metabolic Regulation

Background:

  • Cholesterol metabolism is crucial for development and is regulated by specific enzymes.
  • Hepatic cholesterol 7alpha-hydroxylase (CYP7A) and sterol 27-hydroxylase are key enzymes in bile acid synthesis.
  • Understanding the developmental regulation of these enzymes is essential for pediatric and metabolic research.

Purpose of the Study:

  • To investigate the developmental expression and regulation of hepatic CYP7A and sterol 27-hydroxylase activities and mRNA levels in piglets.
  • To determine the impact of nutritional status (suckling, weaning, fasting, refeeding) on these enzymes.
  • To elucidate the regulatory mechanisms controlling CYP7A activity during development.

Main Methods:

  • Measurement of hepatic CYP7A and sterol 27-hydroxylase enzyme activities using microsomes from piglets at various developmental stages.

Related Experiment Videos

  • Quantification of hepatic CYP7A mRNA levels using Northern blot analysis.
  • Analysis of enzyme activity and mRNA levels following periods of fasting and refeeding.

    • Main Results:

    • Hepatic CYP7A activity and mRNA were undetectable in fetal and newborn piglets, increasing significantly by weaning age.
    • Fasting for 14 hours reduced CYP7A activity and mRNA by over 80%, with delayed recovery after refeeding.
    • Sterol 27-hydroxylase activity was also low at birth but increased by 21 days and showed rapid recovery after refeeding, unlike CYP7A.

    Conclusions:

    • Developmental regulation of hepatic CYP7A activity in piglets is primarily controlled at the pretranslational level.
    • Nutritional status, particularly fasting and refeeding, significantly impacts CYP7A expression and activity.
    • The distinct regulation patterns of CYP7A and sterol 27-hydroxylase highlight their specific roles in cholesterol metabolism during development.