Microsatellite instability and mutation of mitochondrial and nuclear DNA in gastric carcinoma
- W Habano 1, T Sugai , S I Nakamura
- 1Division of Pathology, Central Clinical Laboratory, School of Medicine, Iwate Medical University, Morioka, Japan.
- 0Division of Pathology, Central Clinical Laboratory, School of Medicine, Iwate Medical University, Morioka, Japan.
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View abstract on PubMed
Summary
This summary is machine-generated.Mitochondrial DNA instability (mtMSI) and mutations occur in gastric cancers, particularly the intestinal type. This instability is linked to nuclear DNA instability and p53 gene alterations, suggesting a role in tumorigenesis.
Area Of Science
- Oncology
- Molecular Biology
- Genetics
Background
- Mitochondrial DNA microsatellite instability (mtMSI) is known in colorectal cancers.
- Its presence and clinical significance in gastric carcinomas were previously undetermined.
Purpose Of The Study
- To investigate mitochondrial MSI (mtMSI) and mutations in gastric carcinomas.
- To correlate mtMSI with clinicopathologic features and nuclear DNA instability.
Main Methods
- Analysis of 62 gastric carcinomas (intestinal and diffuse types).
- Examination of mitochondrial gene mutations and mtMSI using PCR-SSCP.
- Assessment of nuclear DNA MSI (nMSI) and p53 gene mutations.
Main Results
- The mtMSI phenotype was observed in 16% of gastric carcinomas.
- Mitochondrial gene mutations were found in 5 cases, 4 with mtMSI.
- mtMSI and nMSI showed a positive correlation, frequently detected in intestinal-type gastric cancers.
Conclusions
- Mitochondrial gene mutations associated with mtMSI may contribute to intestinal-type gastric cancer development.
- Clinicopathologic profiles differ between intestinal and diffuse type gastric carcinomas regarding mtMSI and nMSI.
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