Related Experiment Video
Updated: Jul 21, 2026
12:19
Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
Cyclin D2 is essential for BCR-mediated proliferation and CD5 B cell development.
1Department of Immunology, DNAX Research Institute, Palo Alto, CA 94304, USA.
International Immunology
|April 28, 2000
Summary
Cyclin D2 is essential for B lymphocyte proliferation, particularly after B cell receptor activation. Its absence significantly reduces the CD5 B cell population, impacting immune responses.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- B lymphocyte proliferation is regulated by cell cycle proteins, including cyclins D2 and D3.
- Cyclins D2 and D3 are thought to have overlapping roles in cell cycle control.
- Understanding the specific functions of individual cyclins is crucial for deciphering B cell regulation.
Purpose of the Study:
- To investigate the specific role of cyclin D2 in B lymphocyte proliferation.
- To determine if cyclin D2 has distinct functions compared to cyclin D3 in B cells.
- To analyze the impact of cyclin D2 deficiency on B cell development and activation.
Main Methods:
- Analysis of B cells from cyclin D2 knockout mice (cyclin D2-/-).
- Assessment of B cell proliferation in response to various stimuli, including B cell receptor (BCR) crosslinking, CD40 stimulation, and lipopolysaccharide (LPS).
- Evaluation of B cell development, including conventional B cells and the CD5 B cell subset.
Main Results:
- Cyclin D2 is specifically required for BCR-induced B cell proliferation.
- Proliferation induced by CD40 or LPS is not dependent on cyclin D2.
- Conventional B cell development is unaffected in cyclin D2-deficient mice.
- The CD5 B cell compartment is significantly reduced in cyclin D2 knockout mice.
Conclusions:
- Cyclin D2 plays a critical, non-redundant role in BCR-mediated B cell proliferation.
- Cyclin D2 is important for the development and/or maintenance of the CD5 B cell population.
- These findings highlight a specific requirement for cyclin D2 in adaptive immune responses mediated by B cells.
More Related Videos
Related Concept Videos
01:45Positive Regulator Molecules
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
02:39Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
02:34Inhibition of Cdk Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
02:34Inhibition of CDK Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...