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Mitochondrial ATPase 6 gene expression in unfertilized oocytes and cleavage-stage embryos
Objective:
To compare the level of mitochondrial ATPase 6 gene expression in unfertilized oocytes and cleavage-stage embryos.
Design:
Reverse transcription polymerase chain reaction was performed in unfertilized oocytes and cleavage-stage embryos derived from tripronucleate embryos to determine ATPase 6 gene expression.
Setting:
Department of Obstetrics and Gynecology, Human Genetics Laboratory, Infertility Medical Center of CHA General Hospital, College of Medicine, Pochon CHA University, Seoul, Korea.
Patient(S):
Oocytes were obtained from infertile couples undergoing in vitro fertilization.
Intervention(S):
Unfertilized oocytes collected at 48 hours after retrieval and cleavage-stage embryos derived from tripronucleate embryos were prepared for evaluation of mitochondrial gene expression.
Main Outcome Measure(S):
Comparison of ATPase 6 gene expression by using single-cell reverse transcription polymerase chain reaction.
Result(S):
Expression of unfertilized oocytes decreased compared with early cleavage-stage embryos.
Conclusion(S):
Our findings of decreased ATPase 6 expression in unfertilized oocytes suggest that there may be a decrease in the mitochondrial functional capacity of oxidative phosphorylation.