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An experimental vaccine providing heterologous protection for Leishmania species in murine model

M A Bebars1, A O el Serougi, K M Makled

  • 1Department of Parasitology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.

Journal of the Egyptian Society of Parasitology
|April 29, 2000
PubMed

Five groups each of 20 BALB/C mice were immunized by heat killed L. donovani promastigotes. Two groups served as control. Each group of mice was subdivided into 2 equal subgroups and challenged with either L. donovani or L. major. The results revealed a high cross reactivity paralleled by a high cross protection between L. donovani and L. major. Heat killed L. donovani promastigotes super-passed other tested SDS-PAGE fractionated and eluted L. d. fractions identified by immune BALB/c mice in providing protection against both L. donovani and L. major. The percentage of protection (reduction in parasite burden) offered by each vaccine was as follows: 80.21%, 36.11%, 9.29%, 33.44% and 17.48% respectively against challenge infection with L. donovani and 3.012 x 10(4), 1.095 x 10(4), 6.054 x 10(2), 2.409 x 10(4), and 3.34 x 10(2), respectively against challenge infection with L. major.

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