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Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy
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Freeze-drying method for stable preservation in cellular specimens for the detection of apoptotic cells

T Okuyama1, S Matsuo, D Nagamachi

  • 1Second Department of Pathology, Kobe University School of Medicine, Hyogo, Japan.

Diagnostic Cytopathology
|April 29, 2000
PubMed

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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...