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Related Experiment Videos

c-Jun and p53 activity is modulated by SUMO-1 modification.

S Muller1, M Berger, F Lehembre

  • 1Unité de Recombinaison et Expression Génétique, INSERM Unité 163, Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris Cedex 15, France.

The Journal of Biological Chemistry
|May 2, 2000
PubMed
Summary

Small ubiquitin-related modifier 1 (SUMO-1) modifies transcription factors c-Jun and p53, impacting their activity and function. SUMO-1 conjugation negatively regulates c-Jun and is important for p53

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Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Biochemistry

Background:

  • SUMO-1 covalently modifies proteins like IkappaBalpha, antagonizing ubiquitination and degradation.
  • Transcription factors c-Jun and p53 are known targets of ubiquitination.

Purpose of the Study:

  • To identify new substrates for SUMO-1 modification.
  • To investigate the functional consequences of SUMO-1 conjugation on c-Jun and p53.

Main Methods:

  • In vitro and in vivo assays to detect SUMO-1 conjugation.
  • Site-directed mutagenesis to create SUMO-1-deficient mutants (c-JunK229R, p53 mutant).
  • Reporter gene assays to assess transactivation potential and apoptotic activity.

Main Results:

Related Experiment Videos

  • c-Jun and p53 are identified as novel SUMO-1 substrates.
  • SUMO-1 modification of c-Jun occurs at Lys-229 and negatively regulates its transactivation potential.
  • SUMO-1 modification of p53 at Lys-386 is important for its apoptotic activity.

Conclusions:

  • SUMO-1 conjugation pathway is linked to the regulation of transcription factors c-Jun and p53.
  • SUMO-1 acts as a negative regulator of c-Jun activity.
  • SUMO-1 modification plays a role in the biological activity of p53.