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Direct PCR of Cryptococcus neoformans MATalpha and MATa pheromones to determine mating type, ploidy, and variety: a
S Chaturvedi1, B Rodeghier, J Fan
1Mycology Laboratory, Wadsworth Center, New York State Department of Health, Albany, New York, USA.
Journal of Clinical Microbiology
|May 2, 2000
Abstract:
Cryptococcus neoformans MATalpha and MATa pheromones were amplified by direct PCR. Nucleotide sequence analyses revealed unique restriction enzyme sites. Sixty strains were used to devise a restriction fragment length polymorphism typing scheme that yielded three variety-specific patterns. Additionally, pheromone-specific PCR allowed easier identification of diploid C. neoformans strains than flow cytometry.