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Related Experiment Videos

Altered Th1/Th2 commitment in human CD4+ T cells with ageing.

S Sakata-Kaneko1, Y Wakatsuki, Y Matsunaga

  • 1Department of Clinical Bio-regulatory Science, Graduate School of Medicine, Kyoto University, Kyoto, Japan.

Clinical and Experimental Immunology
|May 3, 2000
PubMed
Summary
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Aging immune systems show altered helper T cell function. Older adults have more CD4+ T cells producing interferon-gamma (IFN-gamma), indicating a shift towards Th1 responses with age.

Area of Science:

  • Immunology
  • Gerontology
  • Cellular Biology

Background:

  • The human immune system undergoes continuous age-related changes.
  • Functional alterations in the aging immune system may stem from altered helper T cell regulation.

Purpose of the Study:

  • To comparatively analyze the function of CD4+ T cells in young and elderly healthy volunteers.
  • To investigate age-associated changes in T cell cytokine production and surface marker expression.

Main Methods:

  • Peripheral blood CD4+ T cells from young and old volunteers were isolated.
  • Cells were activated using phorbol myristate acetate/ionomycin and a co-culture system (TCR/CD3 and CD28 stimulation).
  • Interferon-gamma (IFN-gamma), Interleukin-2 (IL-2), and Interleukin-4 (IL-4) production was measured. Surface marker expression (CD26, CD40L, LFA-1, CD30) was analyzed.

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Main Results:

  • A higher proportion of CD4+ T cells produced IFN-gamma in older subjects post-activation.
  • Activated CD4+ T cells from older subjects secreted more IFN-gamma and IL-2, but less IL-4, compared to younger subjects.
  • Expression of CD26, CD40L, and LFA-1 was elevated, while CD30 was reduced on CD4+ T cells from older individuals.

Conclusions:

  • Aging influences CD4+ T cell function, promoting a shift towards T-helper 1 (Th1) cell responses.
  • The microenvironment in older individuals may favor the development of Th1-committed CD4+ T cells.
  • These findings highlight age-specific immune cell programming and potential implications for immune function in the elderly.