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Related Concept Videos

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Related Experiment Video

Updated: Jun 19, 2026

A Fluorescence-based Exonuclease Assay to Characterize DmWRNexo, Orthologue of Human Progeroid WRN Exonuclease, and Its Application to Other Nucleases
06:10

A Fluorescence-based Exonuclease Assay to Characterize DmWRNexo, Orthologue of Human Progeroid WRN Exonuclease, and Its Application to Other Nucleases

Published on: December 23, 2013

Different deoxyribonucleases in human lymphocytes.

E J Zöllner, W Helm, R K Zahn

    Nucleic Acids Research
    |August 1, 1974
    PubMed
    Summary
    This summary is machine-generated.

    Researchers characterized deoxyribonuclease (DNase) activities in human lymphocytes using micro-disc-electrophoresis. Four distinct DNase groups were identified, differing in mobility, substrate preference, and optimal conditions, providing new insights into DNA regulation.

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    Last Updated: Jun 19, 2026

    A Fluorescence-based Exonuclease Assay to Characterize DmWRNexo, Orthologue of Human Progeroid WRN Exonuclease, and Its Application to Other Nucleases
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    Published on: December 23, 2013

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    Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes
    05:34

    Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes

    Published on: July 14, 2023

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Immunology

    Background:

    • Deoxyribonucleases (DNases) play crucial roles in DNA metabolism and cellular processes.
    • Understanding the specific DNase activities within human lymphocytes is essential for comprehending immune cell function and DNA repair mechanisms.

    Purpose of the Study:

    • To characterize the distribution and properties of deoxyribonuclease activities in human lymphocytes.
    • To differentiate and define distinct DNase groups based on their biochemical characteristics.

    Main Methods:

    • Micro-disc-electrophoresis was employed to separate and analyze DNase activities.
    • Enzyme kinetics, substrate specificity, and optimal pH conditions were assessed for characterized DNase fractions.

    Main Results:

    • Four distinct groups of deoxyribonuclease activities were identified in human lymphocytes.
    • Two alkaline DNase activities were characterized: DNase I and a distinct DNase with a pH optimum of 9.0, preferring denatured DNA and independent of divalent cations.
    • Acidic DNase fractions were further subdivided into two groups, exhibiting differential activity towards native and denatured DNA, and influenced by succinate presence and pH.

    Conclusions:

    • Human lymphocytes possess a complex array of deoxyribonuclease activities with distinct biochemical properties.
    • These findings contribute to a deeper understanding of the enzymatic machinery involved in DNA processing within lymphocytes.