Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

'DNA snapback' peptides.

R L Novak, J Dohnal

    Nucleic Acids Research
    |June 1, 1974
    PubMed
    Summary
    This summary is machine-generated.

    Certain peptides can help denatured calf thymus DNA spontaneously renature. Researchers found a correlation between peptide thermal stabilization of DNA and their renaturation catalysis ability.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Regulation of veterinary point-of-care testing in the European Union, the United States of America and Japan.

    Revue scientifique et technique (International Office of Epizootics)·2022
    Same author

    Coupling between magnetic and optical properties of stable Au-Fe solid solution nanoparticles.

    Nanotechnology·2010
    Same author

    Adenoviral-mediated Rybp expression promotes tumor cell-specific apoptosis.

    Cancer gene therapy·2008
    Same author

    Using of HPLC coupled with coulometric detector for the determination of biotin in pharmaceuticals.

    Journal of pharmaceutical and biomedical analysis·2007
    Same author

    Investigating the antiproliferative activity of quinoline-5,8-diones and styrylquinolinecarboxylic acids on tumor cell lines.

    Bioorganic & medicinal chemistry letters·2007
    Same author

    Use of the zirconia-based stationary phase for separation of ibuprofen and its impurities.

    Journal of pharmaceutical and biomedical analysis·2005
    Same journal

    Correction to 'New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress'.

    Nucleic acids research·2026
    Same journal

    VeloRM: disentangling pre- and post-splicing RNA modification dynamics at single-cell resolution.

    Nucleic acids research·2026
    Same journal

    Accessibility of telomeric overhangs to stabilizing small-molecule ligands.

    Nucleic acids research·2026
    Same journal

    Multivalent interactions mediate SNAIL transcription factor stimulation of the nucleosome deacetylase activity of the CoREST complex.

    Nucleic acids research·2026
    Same journal

    Genome-wide mapping of DNA G-quadruplexes in Trypanosoma brucei chromatin reveals enrichment in coding regions and transcription start sites.

    Nucleic acids research·2026
    Same journal

    Correction to 'The Gene Ontology knowledgebase in 2026'.

    Nucleic acids research·2026
    See all related articles

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Biophysics

    Background:

    • DNA denaturation and renaturation are fundamental processes in molecular biology.
    • Peptides are known to interact with DNA, influencing its structure and function.
    • Understanding DNA renaturation mechanisms is crucial for various biotechnological applications.

    Purpose of the Study:

    • To investigate the spontaneous renaturation of heat-denatured calf thymus DNA in a peptide-DNA complex.
    • To determine the role of specific peptides in catalyzing DNA strand renaturation.
    • To explore the relationship between peptide-induced DNA thermal stabilization and renaturation catalysis.

    Main Methods:

    • Thermal denaturation studies of (Tyr-Gly-Tyr-Gly-Tyr)-DNA complex.
    • Verification of DNA double-strandness using Neurospora endonuclease and hydroxyapatite chromatography.

    Related Experiment Videos

  • Analysis of Guanine-Cytosine (GC) content in renatured DNA.
  • Assessment of renaturation catalysis by tryptophanyl-glycyl and histidyl-glycyl peptides.

    • Main Results:

    • 10-15% spontaneous renaturation of heat-denatured calf thymus DNA was observed.
    • Renatured DNA exhibited increased Guanine-Cytosine (GC) content (56%) compared to whole thymus DNA (41%).
    • Alternating tryptophanyl-glycyl and histidyl-glycyl peptides demonstrated catalytic activity in DNA renaturation.
    • A linear correlation was established between peptide-mediated thermal stabilization of DNA and their renaturation catalytic ability.

    Conclusions:

    • Specific peptides can promote spontaneous renaturation of denatured DNA strands.
    • The Guanine-Cytosine (GC) rich regions of DNA appear to preferentially renature.
    • Peptide-DNA interactions play a significant role in modulating DNA renaturation kinetics and stability.