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Hybrid isolation by recovery of RNA-DNA hybrids from agar using S1 nuclease
Nucleic Acids Research
|June 1, 1974
Abstract:
A method for recovering RNA-DNA hybrids from agar employing a single strand specific nuclease is described. The procedure is suitable for large scale isolations, and immobilization of the DNA in agar prior to hybridization allows a high yield of hybrid without interference by DNA reannealing.