Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Memory CD8+ T cells in HIV infection.

A J McMichael1, G Ogg, J Wilson

  • 1MRC Human Immunology Unit, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK. andrew.mcmichael@clinical-medicine.oxford.ac.uk

Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
|May 4, 2000
PubMed
Summary

Cytotoxic T lymphocytes (CTLs) are crucial for controlling persistent HIV infection. Their numbers and function may decline with HIV-induced CD4+ T helper cell depletion, potentially contributing to AIDS development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

British Association of Dermatologists guidelines for the management of people with chronic urticaria 2021.

The British journal of dermatology·2021
Same author

Anticholinergic drug use and risk of mortality for people with dementia in Northern Ireland.

Aging & mental health·2020
Same author

Association of cicatricial alopecia with chemical hair straightening.

The British journal of dermatology·2019
Same author

Deficiency of filaggrin regulates endogenous cysteine protease activity, leading to impaired skin barrier function.

Clinical and experimental dermatology·2017
Same author

Lipid-specific T cells and the skin.

The British journal of dermatology·2016
Same author

Pseudofolliculitis barbae: understanding the condition and the role of facial grooming.

International journal of cosmetic science·2016

Area of Science:

  • Immunology
  • Virology
  • Cellular Biology

Background:

  • Cytotoxic T lymphocytes (CTLs) are vital for managing persistent viral infections, including Human Immunodeficiency Virus (HIV).
  • The characteristics of the CTL response in HIV infection mirror those seen in other chronic viral diseases.
  • A significant proportion of CD8+ T cells in HIV-infected individuals are specific for dominant viral epitopes.

Purpose of the Study:

  • To investigate the role and characteristics of CTLs in persistent HIV infection.
  • To understand the discrepancy between CTL numbers measured by tetramer assays and limiting dilution assays.
  • To explore the potential impact of CD4+ T helper cell depletion on CTL function and replacement in HIV infection.

Main Methods:

  • Quantification of HIV-specific CD8+ T cells using HLA class I peptide tetramer assays.

Related Experiment Videos

  • Comparison with results from limiting dilution assays to assess CTL proliferation capacity.
  • Assessment of cytokine production (e.g., interferon-gamma) and cytotoxic markers (e.g., perforin) in tetramer-positive T cells.

    • Main Results:

    • Tetramer assays detect higher frequencies of HIV-specific CD8+ T cells (0.1–1.0%) compared to limiting dilution assays.
    • This discrepancy may be due to the inability of some HIV-specific CTLs to undergo sufficient divisions for detection by limiting dilution.
    • Tetramer-positive T cells demonstrate functional markers like interferon-gamma production and perforin expression, indicating probable cytotoxic activity.

    Conclusions:

    • HIV-specific CTLs are abundant and likely functional during chronic infection.
    • The inability of some CTLs to divide may explain lower counts in traditional assays.
    • Impaired CTL replacement, potentially linked to CD4+ T cell depletion by HIV, could be a key factor in the progression to Acquired Immunodeficiency Syndrome (AIDS).