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Related Experiment Videos

Fluorescein in the human optic disc,.

H Goldman

    Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie. Albrecht Von Graefe'S Archive for Clinical and Experimental Ophthalmology
    |January 1, 1975
    PubMed
    Summary
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    Even high intraocular pressure cannot fully empty optic disc capillaries. Fluorescein primarily highlights plasma flow in vessels, not overall disc circulation, making it unsuitable for accurate disc circulation measurement.

    Area of Science:

    • Ophthalmology
    • Vascular Biology
    • Medical Imaging

    Background:

    • Intravenous fluorescein angiography is a key tool in ophthalmology.
    • Understanding fluorescein dye behavior in ocular vasculature is crucial for interpreting imaging results.
    • The optic disc's unique microcirculation presents challenges for accurate assessment.

    Purpose of the Study:

    • To investigate the behavior of fluorescein dye within the optic disc vasculature under varying intraocular pressure conditions.
    • To determine the primary source of fluorescence observed in retinal and papillary vessels after fluorescein injection.
    • To evaluate the suitability of fluorescein angiography for measuring optic disc blood circulation.

    Main Methods:

    • Globe compression to elevated intraocular pressures exceeding systolic ophthalmic artery pressure.

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  • Intravenous fluorescein injection to visualize retinal and optic disc vasculature.
  • Analysis of fluorescein dye distribution and diffusion patterns within the optic disc and surrounding tissues.

    • Main Results:

    • Globe compression did not completely eliminate capillary blood in the optic disc.
    • Fluorescence primarily originated from the plasma layer within vessels, not red blood cells.
    • Smaller vessels showed fluorescence intensity comparable to larger ones, and extravasated dye appeared as bright patches.
    • Fluorescein diffused into the optic disc tissue mainly from adjacent structures like the uvea and sclera.
    • Optic disc circulation was reduced more significantly than retinal vessel circulation during compression.

    Conclusions:

    • Fluorescein angiography visualizes plasma flow and leakage, not necessarily total blood volume or circulation in the optic disc.
    • The diffusion characteristics of fluorescein limit its utility for precise quantification of optic disc blood flow.
    • Fluorescein angiography is not the optimal method for measuring optic disc circulation due to these limitations.