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Updated: Aug 2, 2026
Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies
Published on: February 16, 2010
Rapid purification of fumonisins and their hydrolysis products with solid-phase extraction columns
1Mycotoxin Research, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL 61604, USA. polingsm@mail.ncaur.usda.gov
Abstract:
Fumonisins B(3) and B(4) (FB(3) and FB(4)) were recovered from the 50:50 acetonitrile/water extract of corn cultures of a strain of Fusarium moniliforme that does not make FB(1) or FB(2) by stirring the extract with IRA-68, a weak anion-exchange resin. The fumonisins were desorbed with 5% acetic acid in the same solvent. After dilution with water, the desorbed fumonisins were separated into FB(3) (FB(3) and FA(3)) and FB(4) (FB(4), FC(4), and FA(4)) fractions with a tC(18) solid-phase extraction (SPE) cartridge. The FB(3) fraction was then separated into FB(3) and FA(3) by using an NH(2) SPE cartridge and eluting with 5% acetic acid and increasing amounts of acetonitrile in water. Finally, FB(1) and FA(3) were hydrolyzed with calcium hydroxide. After recovery from the reaction mixture using a tC(18) cartridge, the hydrolyzed and partially hydrolyzed analogues were separated and the unreacted fumonisins recovered by using an NH(2) cartridge, initially in the normal-phase mode with increasing amounts of water in acetonitrile and then in the reversed-phase mode after the addition of 5% acetic acid to the solvent and eluting in the reverse order.
Insights
This study details a method for isolating fumonisins B(3) and B(4) from Fusarium moniliforme corn cultures. The process utilizes anion-exchange and solid-phase extraction techniques for effective separation and recovery of these mycotoxins.
Area of Science:
- Mycology
- Analytical Chemistry
- Food Safety
Background:
- Fumonisins are mycotoxins produced by Fusarium species, posing risks to food and feed safety.
- Certain Fusarium moniliforme strains produce less common fumonisins like FB(3) and FB(4), requiring specific detection methods.
Purpose of the Study:
- To develop and describe a purification strategy for fumonisins B(3) and B(4) from fungal cultures.
- To enable the isolation of specific fumonisins for further toxicological or analytical studies.
Main Methods:
- Extraction of fumonisins from corn cultures using acetonitrile/water.
- Purification using IRA-68 anion-exchange resin and subsequent desorption with acetic acid.
- Separation of fumonisin fractions using C18 and NH2 solid-phase extraction (SPE) cartridges.
- Hydrolysis of specific fumonisins followed by further purification and recovery.
Main Results:
- Successful isolation of fumonisins B(3) and B(4) from a Fusarium moniliforme strain that does not produce FB(1) or FB(2).
- Demonstrated sequential purification steps involving different SPE cartridges to achieve separation of individual fumonisin analogues.
- Recovered unreacted fumonisins and partially hydrolyzed analogues after chemical treatment.
Conclusions:
- The described method provides an effective means for isolating and purifying fumonisins B(3) and B(4).
- This technique is valuable for obtaining pure standards of less common fumonisins for research and regulatory purposes.
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