Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enhanced organically bound selenium yeast production by fed-batch fermentation.

A Demirci1, A L Pometto, D J Cox

  • 1Department of Food Science and Human Nutrition, Iowa State University, Ames 50011, USA.

Journal of Agricultural and Food Chemistry
|May 4, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Piezosurgery versus conventional rotary surgery for impacted third molars: A randomised, split-mouth, clinical pilot trial.

Medicina oral, patologia oral y cirugia bucal·2023
Same author

Comparison of immunogenicity for Sinovac-CoronaVac vaccine vs. natural infection during cancer treatment.

European review for medical and pharmacological sciences·2023
Same author

The significance of a novel inflammatory biomarker, presepsin, in predicting disease prognosis in patients with COVID-19.

European review for medical and pharmacological sciences·2022
Same author

The role of neutrophil albumin ratio in predicting the stage of non-small cell lung cancer.

European review for medical and pharmacological sciences·2022
Same author

High-precision measurement of the <i>W</i> boson mass with the CDF II detector.

Science (New York, N.Y.)·2022
Same author

Evaluating the effect of tumor size and sidedness on prognosis in stage 2 colon cancer: a retrospective population study.

European review for medical and pharmacological sciences·2022

This study optimized Saccharomyces cerevisiae fermentation for higher organically bound selenium production. Low selenium concentrations and adapted strains yielded the most selenium-rich biomass, crucial for nutritional supplements.

Area of Science:

  • Biotechnology and Industrial Microbiology
  • Biochemistry and Nutritional Science

Background:

  • Saccharomyces cerevisiae is a key microorganism for industrial applications.
  • Organic selenium enrichment in yeast biomass is valuable for human and animal nutrition.
  • Optimizing fermentation conditions is essential for maximizing selenium incorporation.

Purpose of the Study:

  • To develop and optimize a fed-batch fermentation protocol for enhanced production of organically bound selenium in Saccharomyces cerevisiae.
  • To investigate the impact of different sodium selenate concentrations and addition methods (single dose vs. continuous) on yeast biomass and selenium content.
  • To evaluate the performance of adapted Saccharomyces cerevisiae strains under optimized conditions.

Main Methods:

  • Fed-batch fermentation of Saccharomyces cerevisiae using cane molasses as a feeding medium.

Related Experiment Videos

  • Application of two sodium selenate concentrations (63.2 g/L and 31.6 g/L) via single-dose or continuous addition.
  • Cultivation of two adapted Saccharomyces cerevisiae strains under low selenium concentration conditions.

    • Main Results:

    • High sodium selenate concentration (63.2 g/L) yielded biomass concentrations of 24-40 g/L with selenium levels of 1382-1491 µg/g dry biomass.
    • Low sodium selenate concentration (31.6 g/L) resulted in higher biomass (37-45 g/L) and significantly higher selenium content (2495-2846 µg/g dry biomass).
    • Adapted strains with low selenium concentration achieved >3000 µg/g dry biomass, albeit with lower biomass concentration (< or =32 g/L).

    Conclusions:

    • Fed-batch fermentation is an effective strategy for enhancing organically bound selenium production in Saccharomyces cerevisiae.
    • Lower sodium selenate concentrations, particularly with continuous feeding or using adapted strains, are more effective for achieving high selenium enrichment in yeast biomass.
    • The findings provide a basis for scalable production of selenium-enriched yeast for nutritional applications.