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Sequestrated thrombolysis: comparative evaluation in vivo
1Institute for Surgical Research, National Hospital, Oslo, Norway.
Cardiovascular and Interventional Radiology
|May 5, 2000
Summary
Transthrombic sequestrated thrombolysis, using concentrated recombinant tissue plasminogen activator (rt-PA), effectively reduced deep vein thrombosis (DVT) in a porcine model. This method shows promise for improving acute DVT treatment outcomes.
Area of Science:
- Vascular Surgery
- Thrombosis Research
- Pharmacology
Background:
- Thrombus lysis depends on local thrombolytic enzyme concentration.
- Acute deep vein thrombosis (DVT) requires effective treatment strategies.
Purpose of the Study:
- To evaluate if perithrombic sequestration of concentrated enzyme solutions accelerates thrombolysis in a porcine DVT model.
- To compare sequestrated thrombolysis with conventional infusion methods.
Main Methods:
- Deep vein thrombosis (DVT) was induced in porcine hind limbs.
- Recombinant tissue plasminogen activator (rt-PA) was administered via conventional infusion (CI), proximal sequestrated thrombolysis (ST-P), or transthrombic sequestrated thrombolysis (ST-T).
- Thrombus mass and residual thrombosis in venous tributaries were measured post-treatment.
Main Results:
- Transthrombic sequestrated thrombolysis (ST-T) normalized residual thrombus mass compared to controls (median RTI 0.50).
- ST-T and ST-P showed significantly lower residual thrombosis in venous tributaries than CI (p=0.04).
- No systemic fibrinogenolysis or significant differences in coagulation parameters were observed across groups.
Conclusions:
- Transthrombic sequestrated thrombolysis demonstrates potential advantages over conventional selective infusion for acute DVT.
- Further refinements are needed to establish ST-T as a viable alternative treatment for DVT.