Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Streptavidin-biotin binding energetics.

P S Stayton1, S Freitag, L A Klumb

  • 1Department of Bioengineering, University of Washington, Seattle 98195, USA. stayton@u.washington.edu

Biomolecular Engineering
|May 5, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microfluidic fluorescence biosensor for quantitative detection of HER2 in serum using an antibody-aptamer sandwich assay.

Talanta·2026
Same author

The collective impact of childhood abuse, psychache, and interpersonal needs on suicidal ideation among individuals with bipolar disorder: A discriminant analysis.

Child abuse & neglect·2023
Same author

Sustained release of a GLP-1 and FGF21 dual agonist from an injectable depot protects mice from obesity and hyperglycemia.

Science advances·2020
Same author

Study on the bio-functionalization of memristive nanowires for optimum memristive biosensors.

Journal of materials chemistry. B·2020
Same author

Is knowledge "half the battle"? The role of depression literacy in help-seeking among a non-clinical sample of adults with currently untreated mental health problems.

Journal of affective disorders·2018
Same author

Individuals with currently untreated mental illness: causal beliefs and readiness to seek help.

Epidemiology and psychiatric sciences·2018
Same journal

Proceedings of Symposium J on Surface Functionalization of Biomaterials at the E-MRS 2006 Fall Conference. September 4-6, 2006. Warsaw, Poland.

Biomolecular engineering·2008
Same journal

Overexpression of GLT1 in fps1DeltagpdDelta mutant for optimum ethanol formation by Saccharomyces cerevisiae.

Biomolecular engineering·2007
Same journal

Fibrils of different collagen types containing immobilised proteoglycans (PGs) as coatings: characterisation and influence on osteoblast behaviour.

Biomolecular engineering·2007
Same journal

Development of a heat shock inducible and inheritable RNAi system in silkworm.

Biomolecular engineering·2007
Same journal

Effect of site-directed PEGylation of trichosanthin on its biological activity, immunogenicity, and pharmacokinetics.

Biomolecular engineering·2007
Same journal

Real-time study of the effect of different stress factors on lactic acid bacteria by electrochemical optical waveguide lightmode spectroscopy.

Biomolecular engineering·2007
See all related articles

The streptavidin-biotin interaction reveals how proteins use enthalpy and entropy for high-affinity binding. This review explores energetics, mutagenesis, and dissociation pathways.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Protein-Ligand Interactions

Background:

  • The streptavidin-biotin system is a model for studying protein-ligand binding energetics.
  • Understanding binding requires analyzing enthalpic and entropic contributions.

Purpose of the Study:

  • To review studies on streptavidin-biotin energetics and dissociation.
  • To elucidate how proteins achieve high affinity and control ligand exit.

Main Methods:

  • Review of site-directed mutagenesis studies.
  • Analysis of biophysical characterization data.
  • Examination of computational modeling results.

Main Results:

  • Detailed energetics of aromatic and hydrogen bonding interactions.

Related Experiment Videos

  • Insights into the activation barrier for streptavidin-biotin dissociation.
  • Exploration of the biotin dissociation pathway.

    • Conclusions:

    • Streptavidin-biotin binding is governed by a balance of enthalpy and entropy.
    • Protein design principles for high affinity and controlled dissociation are highlighted.
    • Understanding ligand exit mechanisms is crucial for protein function.