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Avidin and streptavidin in clinical diagnostics
1Roche Diagnostics GmbH, Labsystems R&D, Rare Reagents, Penzberg, Germany. hartmut.schetters@roche.com
Biomolecular Engineering
|May 5, 2000
Summary
The introduction of the SA/Bi technology enables ultrasensitive cytokine ELISAs, detecting picogram levels with rapid 10-20 minute results. This advancement allows for the measurement of normal base cytokine levels, not just elevated ones, in clinical diagnostics.
Area of Science:
- Clinical diagnostics
- Immunotechnology
Background:
- Traditional immunological tests had limitations in sensitivity and turnaround time.
- Detection of normal base levels for cytokines was not feasible with older assays.
Purpose of the Study:
- To evaluate the feasibility of ultrasensitive tests with acceptable turnaround times using SA/Bi technology.
- To enable the detection of picogram range cytokine levels in clinical diagnostics.
Main Methods:
- Introduction of SA/Bi technology into clinical diagnostic workflows.
- Development of cytokine Enzyme-Linked Immunosorbent Assays (ELISAs) utilizing SA/Bi.
Main Results:
- SA/Bi technology facilitates ultrasensitive tests with acceptable turnaround times.
- Cytokine ELISAs with picogram-level detection limits are now feasible.
- Standard clinical immunological tests now have reduced turnaround times of 10-20 minutes.
Conclusions:
- SA/Bi technology has significantly advanced clinical diagnostics by enabling ultrasensitive cytokine detection.
- The technology allows for the measurement of normal base cytokine levels, improving diagnostic capabilities.
- Rapid turnaround times enhance the clinical utility of immunological assays.