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Related Experiment Videos

Developmental expression of OSP/claudin-11.

J M Bronstein1, K Chen, S Tiwari-Woodruff

  • 1Department of Neurology, UCLA School of Medicine, Los Angeles, California 90024, USA.

Journal of Neuroscience Research
|May 8, 2000
PubMed
Summary

Oligodendrocyte-specific protein (OSP/claudin-11) is crucial for CNS myelin development. Its expression is tightly regulated during development, appearing in oligodendrocytes and testes, suggesting key roles in cell growth and differentiation.

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Disrupted compaction of CNS myelin in an OSP/Claudin-11 and PLP/DM20 double knockout mouse.

Molecular and cellular neurosciences·2005

Area of Science:

  • Neuroscience
  • Cell Biology
  • Developmental Biology

Background:

  • Oligodendrocyte-specific protein (OSP), also known as claudin-11, is a key component of central nervous system (CNS) myelin.
  • It has recently been classified as a member of the claudin family of tight junction proteins.

Purpose of the Study:

  • To investigate the developmental expression patterns of OSP/claudin-11.
  • To understand the role of OSP/claudin-11 in oligodendrocyte development and other cellular processes.

Main Methods:

  • In situ hybridization to detect OSP/claudin-11 mRNA expression.
  • Immunohistochemistry (IH) to localize OSP/claudin-11 protein.
  • Western blot analysis to examine OSP/claudin-11 transcripts.
  • Electron microscopic IH for high-resolution protein localization.

Related Experiment Videos

  • Double-labeling IH with O2-A progenitor cells.

    • Main Results:

    • OSP/claudin-11 mRNA exhibited bimodal expression during development.
    • Prenatal expression was observed in developing meninges, cartilage-adjacent areas, and mesoderm.
    • Postnatal expression was primarily in developing oligodendrocytes and also in testes.
    • Expression begins in early oligodendrocyte progenitor cells and continues through mature oligodendrocytes.
    • Electron microscopy localized OSP/claudin-11 to laminar myelin in the adult CNS.
    • Western blots revealed two developmentally regulated OSP/claudin-11 transcripts.

    Conclusions:

    • OSP/claudin-11 expression is highly regulated throughout development.
    • This protein likely plays a significant role in the growth and differentiation of oligodendrocytes.
    • OSP/claudin-11 may also have important functions in cells outside the CNS.