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Trans-vivo Delayed Type Hypersensitivity Assay for Antigen Specific Regulation
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Published on: May 2, 2013

Transforming growth factor-beta and interleukin-10 subvert alloreactive delayed type hypersensitivity in cardiac

A A Bickerstaff1, A M VanBuskirk, E Wakely

  • 1Department of Surgery, The Ohio State University College of Medicine, Columbus 43210, USA.

Transplantation
|May 8, 2000
PubMed

We have previously reported that temporary treatment of cardiac allograft recipients with gallium nitrate (GN) results in indefinite graft survival, and the inability to mount donor-reactive delayed type hypersensitivity (DTH) responses. We report that antibodies to either transforming growth factor-beta (TGFbeta) or interleukin-10 (IL10) can uncover DTH responses to donor alloantigens in cardiac allograft acceptor mice. The DTH responses uncovered with TGFbeta-reactive antibodies can be blocked by exogenous IL10, and those uncovered with IL10-reactive antibodies can be blocked by exogenous TGFbeta. These data demonstrate that allograft acceptor mice are fully allosensitized, and poised to make donor-reactive cell-mediated immune responses. However, such responses are subverted by a donor alloantigen-dependent mechanism that involves TGFbeta and IL10, which in turn interfere with local cell-mediated immune responses.

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