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Clonal analysis of the human B-cell repertoire using a heteromobility assay
1Aaron Diamond AIDS Center, New York, NY 10016, USA. dtewari@state.pa.us
Clinical and Diagnostic Laboratory Immunology
|May 9, 2000
Abstract:
A heteromobility duplex tracking assay was developed to analyze B-cell clonality. The assay was based on the genetic variability of B-cell immunoglobulin (Ig) sequences. Binding of amplified (Ig) sequences to a single-stranded radiolabeled Ig DNA probe resulted in the formation of heteroduplexes. The mobilities of these heteroduplexes helped to distinguish clonal B cells.
