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Related Experiment Videos

Human lymphocyte subpopulation: early and late rosettes.

D T Yu

    Journal of Immunology (Baltimore, Md. : 1950)
    |July 1, 1975
    PubMed
    Summary

    Early and late sheep red blood cell (SRBC) rosettes of human lymphocytes differ. Late rosettes show more capping, suggesting variations in membrane receptors and sialic acid content influence rosette formation.

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    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Human lymphocytes form rosettes with sheep red blood cells (SRBCs).
    • Two distinct types of rosettes, early and late, have been observed based on formation time.
    • Late rosettes require cold incubation (4°C) for formation.

    Purpose of the Study:

    • To investigate the differences between early and late human lymphocyte-SRBC rosettes.
    • To explore the role of membrane components in the distinct characteristics of these rosettes.

    Main Methods:

    • Separation of early and late rosettes using gradient centrifugation.
    • Analysis of cell capping in both rosette types.
    • Enzymatic treatment (trypsin, AET, neuraminidase) of early rosettes to assess membrane component effects.

    Main Results:

    • Late rosettes exhibited a significantly higher proportion of cell capping compared to early rosettes.
    • Treatment of early rosettes with trypsin, AET, and neuraminidase reduced capping, making them resemble late rosettes.
    • These findings suggest differences in membrane sulphydryl and sialic acid groups.

    Conclusions:

    • The distinct properties of early and late rosettes are likely due to differences in membrane sulphydryl and sialic acid group content.
    • Variations in the distribution of SRBC receptors on the lymphocyte membrane contribute to the observed differences.

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