Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phosphopeptide analysis.

M Quadroni1, P James

  • 1Biomedical Research Center, University of British Columbia, Vancouver, Canada.

EXS
|May 10, 2000
PubMed
Summary
This summary is machine-generated.

This chapter reviews methods for analyzing phosphorylated peptides, focusing on mass spectrometry (MS) techniques for selective detection and sequencing after isolation using chromatography or antibodies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prevalence of molar incisor hypomineralisation and associated factors amongst 8-year-olds in Ireland.

European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry·2025
Same author

Measuring the 3-30-300 rule to help cities meet nature access thresholds.

The Science of the total environment·2023
Same author

Risk factors associated with short-term complications in mandibular fractures: the MANTRA study-a Maxillofacial Trainee Research Collaborative (MTReC).

Oral and maxillofacial surgery·2022
Same author

Out of harm's way: scrotal support for transperineal prostate biopsy.

Annals of the Royal College of Surgeons of England·2022
Same author

Immediate postoperative care on high dependency unit or ward following microvascular free tissue transfer: lessons learnt from a change in practice imposed during the COVID-19 pandemic.

The British journal of oral & maxillofacial surgery·2021
Same author

Impact of Reducing Water Fluoride on Dental Caries and Fluorosis.

Journal of dental research·2020
Same journal

Forensic toxicology.

EXS·2010
Same journal

Biological warfare agents.

EXS·2010
Same journal

Chemical warfare agents.

EXS·2010
Same journal

Drugs of abuse: management of intoxication and antidotes.

EXS·2010
Same journal

Biological testing for drugs of abuse.

EXS·2010
Same journal

Inhalation toxicology.

EXS·2010
See all related articles

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Proteomics

Background:

  • Phosphorylated peptides play crucial roles in cellular signaling.
  • Accurate analysis of phosphopeptides is essential for understanding biological processes.
  • Existing analytical methods require refinement for comprehensive phosphoproteome analysis.

Purpose of the Study:

  • To review and outline current methods for phosphopeptide analysis.
  • To focus on mass spectrometry (MS) based techniques for phosphopeptide detection and sequencing.
  • To provide examples of selective phosphopeptide analysis using MS.

Main Methods:

  • Isolation of phosphopeptides using immobilised metal affinity chromatography, anti-phosphoamino acid antibodies, High Pressure Liquid Chromatography (HPLC), and Thin Layer Chromatography (TLC).

Related Experiment Videos

  • Analysis of isolated peptides via chemical modification followed by Edman degradation.
  • In-depth focus on mass spectrometry (MS) methods for phosphopeptide analysis.

    • Main Results:

    • Demonstration of various isolation techniques for phosphopeptides.
    • Comparison of analytical approaches including Edman degradation and MS.
    • Illustrative examples showcasing the selective detection and sequencing capabilities of MS for phosphopeptides.

    Conclusions:

    • Mass spectrometry offers powerful capabilities for selective phosphopeptide detection and sequencing.
    • A combination of effective isolation and advanced MS techniques is key for phosphoproteomics.
    • Further development in MS methods will enhance the understanding of phosphorylation-dependent biological pathways.