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Chromosome Preparation From Cultured Cells
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Chromosome Preparation From Cultured Cells

Published on: January 28, 2014

Clonal chromosomal abnormalities identified by interphase fluorescence in situ hybridization on collagen cultured

D Le Tessier, J M Dupont, S Fichelson

    Leukemia
    |May 10, 2000
    PubMed
    Abstract

    No abstract available in PubMed .

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    Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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