[Gene examination methods (detection and genotyping of resistant genes)--MRSA]

Molecular diagnosis of MRSA infection including methods of detecting drag-resistant gene and genotyping is described. In Japan, MRSA is the major pathogen for nosocomial infections. For symptomatic patients with MRSA infection, rapid detection of mecA and spa gene by PCR is quite critical to their prognosis. In cases of carry-over contamination of PCR products and mixed infection with coagulase-negative Staphylococci, take precautions are needed while working with this examination. Analysis by restriction length polymorphisms using pulsed-field gel electrophoresis (PFGE-RFLPs) for MRSA nosocomial infection is also described.