Determination of Lufenuron in canine skin layers by radioluminography

In a bioavailability study in dogs we investigated the quantity of [14C]-Lufenuron and its metabolites in the skin by radioluminography. Two Beagle dogs were orally administered 10 mg [14C]-Lufenuron per kg body weight. 21 days later they were euthanized. Skin specimens were taken at 6 different sites. Sections of these skin specimens were exposed on a phosphorus imaging plate and the radiolabeled Lufenuron content visualized. Radioluminographical analysis showed that [14C]-Lufenuron appeared in the subcutaneous layer of the skin. We did not discover any activity on the skin surface.