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Interaction between NiCl2, and nucleobases, nucleosides and nucleotides
1Department of Biomedical Sciences, Faculty of Health Sciences, The University of Sydney, NSW, Australia. f.huq@cchs.usyd.edu.au
Journal of Inorganic Biochemistry
|May 11, 2000
Summary
Nickel(II) chloride interacts with guanine, adenine, and their derivatives, forming specific 1:1 complexes. These nickel complexes primarily involve bonding with the N7 atom of adenine and guanine.
Area of Science:
- Coordination Chemistry
- Biophysical Chemistry
- Spectroscopy
Background:
- Nickel(II) chloride (NiCl2) is a common inorganic compound with known biological relevance.
- Nucleobases, nucleosides, and nucleotides are fundamental building blocks of nucleic acids.
Purpose of the Study:
- To investigate the interaction between Nickel(II) ions and key biological molecules like nucleobases, nucleosides, and nucleotides.
- To elucidate the structural and spectral properties of the resulting complexes.
Main Methods:
- UV-Vis difference spectrophotometry
- Graphite furnace atomic absorption spectrophotometry
- Infrared (IR) spectroscopy
- High-pressure liquid chromatography (HPLC)
- Continuous variation technique
- Molecular modeling (HyperChem 5)
Main Results:
- 1:1 complexes were formed between Ni2+ and guanine, 2'-dGMP, GMP, adenine, and AMP.
- Complexes formed include Ni(Guanine)(H2O)5, Ni(2'-dGMP)(H2O)5, Ni(GMP)(H2O)5, Ni(Adenine)(H2O)5, and Ni(AMP)(H2O)5.
- Molecular modeling confirmed proposed structures and generated electronic/vibrational spectra.
- Ni2+ is proposed to coordinate with the N7 atom of adenine and guanine in these complexes.
Conclusions:
- Nickel(II) ions form stable 1:1 complexes with guanine, adenine, and their phosphorylated derivatives.
- The coordination site for Ni2+ is predominantly the N7 atom of the purine ring.
- The study provides insights into the coordination chemistry of nickel with biologically relevant molecules.