New principle of closed system centrifugation. 1956

An object undergoing circular motion, like a race car, is accelerating because it is changing the direction of its velocity. This centrally directed acceleration is called centripetal acceleration. This acceleration acts along the radius of the curved path (thus is also referred to as radial acceleration).
Any acceleration must be produced by some force. Therefore, any force or combination of forces can cause centripetal acceleration. A few examples include the tension in the rope on a...

In 1653, the French philosopher and scientist Blaise Pascal published "Treatise on the Equilibrium of Liquids," which discussed the principles of static fluids. A static fluid is a fluid that is not in motion. When a fluid is not flowing, we say that the fluid is in static equilibrium. If the fluid is water, we say it is in hydrostatic equilibrium. For a fluid in static equilibrium, the net force on any part of the fluid must be zero; otherwise, the fluid will start to flow. Pascal observed...

Bernoulli's equation incorporates how fluid pressure changes across a static, incompressible fluid by equating the kinetic energy contribution to zero. It is also helpful in analyzing horizontal flows in which the gravitational energy density is constant throughout. The latter equation is so useful that it is called Bernoulli's principle. According to Bernoulli's principle, the fluid pressure drops if the speed increases and vice versa.
Bernoulli's principle has several applications. It is used...

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...