Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Role of Ion Channels in Neuronal Computation01:19

The Role of Ion Channels in Neuronal Computation

A postsynaptic neuron usually receives numerous impulses from several other presynaptic neurons. The axon hillock of the postsynaptic neuron integrates all these signals and determines the likelihood of firing an action potential.
Sometimes a single EPSP is strong enough to induce an action potential in the postsynaptic neuron. However, multiple presynaptic inputs must often create EPSPs around the same time for the postsynaptic neuron to be sufficiently depolarized to fire an action potential.
G-Protein Gated Ion Channels01:21

G-Protein Gated Ion Channels

GPCRs are primarily responsible for our sense of smell, taste, and vision.  The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory organs,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stakeholder engagement to identify barriers to implementation and inform the development of point-of-care diagnostics for TB.

IJTLD open·2026
Same author

Geriatric Assessment-guided therapy modification and outcomes in patients with non-metastatic gastroesophageal cancer: a retrospective cohort study.

ESMO gastrointestinal oncology·2026
Same author

The impact of co-occurring tumor suppressor mutations with mEGFR as early indicators of relapse in lung cancer.

ESMO open·2025
Same author

Navigating the labyrinth: Phenotypes of IgG4-related disease.

Journal of postgraduate medicine·2025
Same author

Neoadjuvant Chemotherapy and Low Dose Immunotherapy in Resectable Non-small Cell Lung Cancer: A Multi-center Retrospective Cohort Analysis.

Clinical oncology (Royal College of Radiologists (Great Britain))·2025
Same author

Clinical symptoms and surgical outcome of colloid cysts of the third ventricle: A multicenter retrospective study.

Brain & spine·2025

Related Experiment Video

Updated: Jul 6, 2026

GABA-activated Single-channel and Tonic Currents in Rat Brain Slices
09:59

GABA-activated Single-channel and Tonic Currents in Rat Brain Slices

Published on: July 17, 2011

Ca(2+) channels involved in the generation of the slow afterhyperpolarization in cultured rat hippocampal pyramidal

M Shah1, D G Haylett

  • 1Department of Pharmacology, University College London, 6BT, United Kingdom.

Journal of Neurophysiology
|May 11, 2000
PubMed
Summary
This summary is machine-generated.

Cultured hippocampal pyramidal cells generate slow afterhyperpolarization currents (sI(AHP)). Intracellular calcium rise, involving multiple pathways beyond L-type channels, activates sI(AHP) in these neurons.

More Related Videos

Whole-cell Patch-clamp Recordings from Morphologically- and Neurochemically-identified Hippocampal Interneurons
14:37

Whole-cell Patch-clamp Recordings from Morphologically- and Neurochemically-identified Hippocampal Interneurons

Published on: September 30, 2014

Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit
11:37

Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit

Published on: August 2, 2017

Related Experiment Videos

Last Updated: Jul 6, 2026

GABA-activated Single-channel and Tonic Currents in Rat Brain Slices
09:59

GABA-activated Single-channel and Tonic Currents in Rat Brain Slices

Published on: July 17, 2011

Whole-cell Patch-clamp Recordings from Morphologically- and Neurochemically-identified Hippocampal Interneurons
14:37

Whole-cell Patch-clamp Recordings from Morphologically- and Neurochemically-identified Hippocampal Interneurons

Published on: September 30, 2014

Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit
11:37

Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit

Published on: August 2, 2017

Area of Science:

  • Neuroscience
  • Cell Biology
  • Pharmacology

Background:

  • Short-term cultures of hippocampal pyramidal cells retain key physiological properties.
  • These cultured cells can generate afterhyperpolarizations following action potential trains.

Purpose of the Study:

  • To investigate the ionic mechanisms underlying the slow afterhyperpolarization current (sI(AHP)) in cultured hippocampal pyramidal cells.
  • To characterize the pharmacological properties and calcium channel involvement in sI(AHP) activation.

Main Methods:

  • Perforated-patch recordings from hippocampal pyramidal cells cultured for 8-15 days.
  • Application of various pharmacological agents including apamin, TEA, charybdotoxin, D-tubocurarine, nifedipine, nimodipine, omega-conotoxin GVIA, and ryanodine.
  • Measurement of medium and slow afterhyperpolarization currents (mI(AHP) and sI(AHP)).

Main Results:

  • sI(AHP) in cultured cells exhibited similar kinetics and pharmacology to those in acute slices.
  • sI(AHP) was insensitive to apamin and charybdotoxin but inhibited by D-tubocurarine.
  • L-type calcium channel blockers (nifedipine, nimodipine) partially inhibited sI(AHP), with higher nimodipine concentrations abolishing it.
  • N-type calcium channel inhibition by omega-conotoxin GVIA significantly reduced sI(AHP).
  • Ryanodine also reduced sI(AHP), suggesting a role for calcium-induced calcium release.

Conclusions:

  • Activation of sI(AHP) in cultured hippocampal pyramidal cells is mediated by an increase in intracellular calcium.
  • Multiple calcium influx pathways, including L-type and N-type channels, contribute to sI(AHP) activation.
  • Calcium-induced calcium release may also play a role in sI(AHP) generation.