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Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
11:32

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

Published on: December 4, 2010

Nuclear export of heat shock and non-heat-shock mRNA occurs via similar pathways

I E Vainberg1, K Dower, M Rosbash

  • 1Department of Biology, Howard Hughes Medical Institute, MS008 Brandeis University, Waltham, Massachusetts 02454, USA.

Molecular and Cellular Biology
|May 11, 2000
PubMed

Insights

Heat shock conditions in yeast do not selectively export heat shock mRNA (hs mRNA). The nucleoporin Rip1p (Nup42p) is essential for general mRNA export, not just hs mRNA export, during heat stress.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Yeast Genetics

Background:

  • Studies in Saccharomyces cerevisiae suggest differential regulation of heat shock mRNA (hs mRNA) and non-hs mRNA nuclear export during stress.
  • Previous research indicated hs mRNA export inhibition at 42°C without the nucleoporin-like protein Rip1p (Nup42p).

Purpose of the Study:

  • To investigate the selective regulation of mRNA nuclear export under heat shock conditions in yeast.
  • To determine the role of Rip1p (Nup42p) in heat shock mRNA (hs mRNA) and non-hs mRNA export.

Main Methods:

  • Analysis of mRNA export in wild-type yeast strains at 42°C.
  • Investigation of mRNA export in strains lacking Rip1p (Nup42p) under heat shock.
  • Examination of potential cis-acting sequences involved in hs mRNA transport.

Main Results:

  • No evidence for selective non-hs mRNA retention or selective hs mRNA export at 42°C in wild-type yeast.
  • hs mRNA export is mediated by the Ran system and general mRNA export factors.
  • Absence of Rip1p (Nup42p) inhibits both non-hs mRNA and hs mRNA export at 42°C.
  • No cis-acting hs mRNA sequences were found to promote transport during heat shock.

Conclusions:

  • A shift to 42°C in the absence of Rip1p (Nup42p) affects a late stage of nuclear export, impacting most or all mRNAs.
  • The data suggest a general role for Rip1p (Nup42p) in mRNA export under heat stress, rather than selective hs mRNA export.

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