Quantitative radioluminography of (125)Iodine whole-body autoradiograms

Radioluminography (RLG) was applied for the quantitation of (125)iodine ((125)I) autoradiograms using the bioimaging analyzer BAS 2000. RLG was performed on 50-microm sections of sagittal whole-body sections of rats and (125)I-radiolabeled erythrocyte calibration scales. Linearity, sensitivity, accuracy, and reproducibility were investigated in comparison to direct (125)I radioactivity measurement. RLG is demonstrated to be a simple, reproducible, and precise analytical tool. However, the spatial resolution of quantitative (125)I RLG is limited by the relatively high average free path of gamma-radiation and X rays.