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Suppression of facilitative glucose transporter 1 mRNA can suppress tumor growth
1First Department of Surgery, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama, Japan. yznog@med.yokohama-cu.ac.jp
Abstract:
We attempted to suppress glucose transporter 1 (GLUT1) expression by transfecting MKN45 cells with cDNA for antisense GLUT1. Glucose transport was significantly decreased in cells with antisense GLUT1 compared with wild-type cells or cells with vector alone. Suppression of GLUT1 mRNA resulted in a decreased number of cells in the S phase. This was accompanied by overexpression of p21 protein. Tumorigenicity in the nude mice injected with antisense GLUT1 expressing cells was significantly slower than in those with wild-type MKN45 cells. These results suggest that antisense GLUT1 mRNA inhibits tumor growth through a G(1) arrest and that expression of antisense GLUT1 mRNA via gene therapy can be used as a tool in the treatment of cancer.
Insights
Researchers suppressed glucose transporter 1 (GLUT1) expression using antisense GLUT1, significantly reducing glucose transport and tumor growth in mice. This suggests gene therapy targeting GLUT1 may offer a new cancer treatment strategy.
Area of Science:
- Molecular Biology
- Cancer Research
- Gene Therapy
Background:
- Glucose transporter 1 (GLUT1) plays a crucial role in cancer cell metabolism and proliferation.
- Targeting GLUT1 is a potential strategy for inhibiting tumor growth.
Purpose of the Study:
- To investigate the effect of suppressing glucose transporter 1 (GLUT1) expression on cancer cell behavior and tumor growth.
- To evaluate the potential of antisense GLUT1 gene therapy for cancer treatment.
Main Methods:
- MKN45 cells were transfected with cDNA for antisense GLUT1 to suppress GLUT1 expression.
- Glucose transport, cell cycle progression, and p21 protein levels were analyzed.
- Tumorigenicity was assessed in nude mice injected with modified MKN45 cells.
Main Results:
- Antisense GLUT1 significantly decreased glucose transport in MKN45 cells compared to controls.
- Suppression of GLUT1 mRNA led to cell cycle arrest in the G1 phase and increased p21 protein.
- Tumor growth was significantly inhibited in mice injected with antisense GLUT1-expressing cells.
Conclusions:
- Antisense GLUT1 mRNA effectively inhibits tumor growth by inducing G1 cell cycle arrest.
- Antisense GLUT1 gene therapy holds promise as a novel therapeutic approach for cancer treatment.