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A peanut seed lipoxygenase responsive to Aspergillus colonization
G B Burow1, H W Gardner, N P Keller
1Department of Plant Pathology and Microbiology, Texas A&M University, College Station 77843-2132, USA.
Plant Molecular Biology
|May 16, 2000
Summary
Lipoxygenase enzymes (LOX) and their hydroperoxy fatty acid products are involved in Aspergillus/seed interactions. A peanut gene, PnLOX1, was cloned and characterized, revealing its role in producing specific hydroperoxides that may increase during infection.
Area of Science:
- Plant biochemistry
- Molecular biology
- Mycology
Background:
- Lipoxygenase (LOX) enzymes and their hydroperoxide products are implicated in Aspergillus/seed interactions.
- These hydroperoxides influence Aspergillus sporulation and aflatoxin gene expression.
Purpose of the Study:
- To investigate the role of seed lipoxygenases at a molecular level.
- To clone and characterize a peanut (Arachis hypogaea L.) seed gene, PnLOX1, involved in these interactions.
Main Methods:
- Nucleotide sequence analysis of the cloned PnLOX1 gene.
- Subcloning the full-length PnLOX1 cDNA into an expression vector.
- Analysis of hydroperoxide products generated by the PnLOX1 enzyme.
Main Results:
- PnLOX1 encodes a 98 kDa protein similar to soybean LOX2.
- PnLOX1 produced a mixture of 9S-HPODE (30%) and 13S-HPODE (70%) at pH 7.
- PnLOX1 is constitutively expressed in immature cotyledons and induced by methyl jasmonate, wounding, and Aspergillus infection in mature cotyledons.
Conclusions:
- PnLOX1 plays a role in the Aspergillus/seed interaction by producing specific hydroperoxides.
- Expression of PnLOX1 in infected cotyledons may increase 9S-HPODE levels, potentially influencing the interaction.