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Comparative genome mapping in the sequence-based era: early experience with human chromosome 7
J W Thomas1, T J Summers, S Q Lee-Lin
1Genome Technology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892 USA.
Genome Research
|May 16, 2000
Summary
Human genome sequence data is refining mouse genome maps, aiding comparative genomics. Analyzing human chromosome 7 sequence identified over 250 mouse genes, enhancing understanding of genome evolution.
Area of Science:
- Comparative Genomics
- Genomics
- Molecular Biology
Background:
- The Human Genome Project's success accelerated genome sequencing efforts for humans and mice.
- Comparative maps and physical maps are crucial for understanding genome organization and evolution.
Purpose of the Study:
- To leverage human chromosome 7 sequence data to refine human-mouse comparative maps.
- To assemble sequence-ready mouse physical maps using human sequence information.
Main Methods:
- Analyzing human chromosome 7 sequence for orthologs of mapped mouse genes to enhance comparative maps.
- Designing mouse-specific probes from human sequence data to screen bacterial artificial chromosome (BAC) libraries for physical mapping.
- Assembling BAC contigs and expanding them using BAC-end sequences.
Main Results:
- Human chromosome 7 sequence analysis more than doubled the number of genes on the human-mouse comparative map.
- Identified at least 20 homologous DNA segments of human chromosome 7 in the mouse genome.
- Assembled over 100 mouse BAC contigs, including over 250 genes, representing approximately 40% of the mouse genome homologous to human chromosome 7.
Conclusions:
- Genomic sequence data from one species directly facilitates comparative genomics and genome evolution studies.
- The integration of human and mouse genomic data provides a powerful approach for genome mapping and analysis.
- This strategy accelerates the assembly of sequence-ready physical maps for complex genomes.