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Phenylalanyl-tRNA synthetase interacts with DNA: studies on activity using deoxyribooligonucleotides
K A Ivanov1, N A Moor, V N Ankilova
1Novosibirsk Institute of Bioorganic Chemistry, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia. ivkon@niboch.nsc.ru
Biochemistry. Biokhimiia
|May 16, 2000
Abstract:
Phenylalanyl-tRNA synthetase from Thermus thermophilus complexes with short (up to 30 nucleotide length) single-stranded DNA fragments more efficiently than with double-stranded fragments. The complexing between DNA and the protein significantly increases with deoxyribooligonucleotide longer than 20 nucleotides. Using affinity labeling, the binding site of DNA was located near the interface of the alpha- and beta-subunits. The binding sites of DNA and tRNAPhe do not overlap. Phenylalanyl-tRNA synthetase from E. coli also binds DNA.